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KMID : 0371320110810040229
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Journal of the Korean Surgical Society
2011 Volume.81 No. 4 p.229 ~ p.234
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Effect of hypertonic saline and macrophage migration inhibitory factor in restoration of T cell dysfunction
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Yoon Young-Hoon
Choi Sung-Hyuk
Hong Yun-Sik
Lee Sung-Woo
Moon Sung-Woo
Cho Han-Jin
Han Cheul
Cheon Young-Jin
Bansal Vishal
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Abstract
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Purpose: Trauma-induced suppression of cellular immune function likely contributes to sepsis, multiple organ dysfunction syndrome and death. T cell proliferation decreases after traumatic stress. The addition of prostaglandin E2 (PGE2), which depresses immune function after hemorrhage and trauma, to T-cells decreases T-cell proliferation; and hypertonic saline restores PGE2-induced T-cell suppression. Recently, it has become apparent that macrophage migration inhibitory factor (MIF) plays a central role in several immune responses, including T-cell proliferation. However, the role of MIF in mediating hypertonic saline (HTS) restoration of T cell dysfunction is unknown. Therefore, we hypothesize that T cell immune restoration by HTS occurs, at least in part, by a MIF-mediated mechanism.
Methods: Jurkat cells were cultured in Roswell Park Memorial Institute media, at a final concentration of 2.5 × 106 cell/mL. The effects of HTS on T-cell proliferation following PGE2-induced suppression were evaluated in Jurkat cells: HTS at 20 or 40 mmol/L above isotonicity was added. MIF levels were determined by enzyme-linked immunosorbent assay and western blot analysis.
Results: PGE2 caused a 15.0% inhibition of Jurkat cell proliferation, as compared to the control. MIF levels decreased in PGE2-suppressed cells, as compared to the control. MIF levels were higher in cells treated with HTS than PGE2-stimulated cells.
Conclusion: The role of HTS in restoring Jurkat cells proliferation suppressed by PGE2, at least in part, should be mediated through a MIF pathway.
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KEYWORD
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Hypertonic solutions, Macrophage Migration-Inhibitory factors, Prostaglandins E, Injuries, T-lymphocytes
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